The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Our specialization servicing. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. The SpectraMax® iD3 and iD5 measure absorbance, fluorescence, and luminescence. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. In addition, the SpectraMax® iD5 can be expanded to include bottom. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. 33 Dramatic aggregation of Alzheimer A b by Cu(II) is induced by conditions representing physiological acidosis . The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. 1. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Total volume (except deep. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published0200-6262. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Summary of Contents for Molecular Devices SpectraMax M2. \ Figure 3. For readers without a cuvette port, simply check the box next to ‘PathCheck’. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. Introduction. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. ; Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Due to elevated security of a Windows 7 64-bit operating system, the USB instrument drivers may not be installed automatically. Other technologies such as imaging. Lift up the lid over the chamber to insert or remove a cuvette. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Download PDF. Example: [Drawer]. When read on microplate readers, the SpectraMax Quant dsDNA Assay Kits enable optimal results and increased assay throughput. We use Spectramax M5 microplate reader in our lab. no. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. 1. microplates or in a cuvette at a. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Figure 1. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. Add to Cart. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. SpectraMax Plus: SpectraMax Plus. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Other features and benefits include: Eliminate changing out filters. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Three low volume 24-well bottom slides. SpectraMax Glo Steady-Luc Reporter Assay Kit. The plate reader can measure multiple readouts, including UV. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Win7 Manual Spectramax-L or StakMax Driver Installation after Initial Install. It also offers a field-upgradeable cellular imaging option and a cooled PMT for low-light sensitivity. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Guides: SpectraMax Mini Installation Guide. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Detection modalities include absorbance (UV-Vis Abs) and. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. In addition, the SpectraMax iD5 can be expanded to. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of. . Cell Cytotoxicity assay procedure. on the SpectraMax iD3 microplate reader Introduction The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. With optimized reagents, option to operate the. We would like to show you a description here but the site won’t allow us. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Page 28 Chapter 5: Read Modes and Read Types 1. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). 305 reference level saturation. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Learn more about its features, specifications, and applications in this user. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Find regional support. Measuring platelet aggregation with a microplate reader. 030 fmol Fluorescence Polarization (FP). Compact designs. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. The SpectraMax Mini Multi-Mode Microplate Reader performs your favorite applications including ELISA, DNA and protein quantitation, cell viability, gene expression with a user-friendly reader. SpectraMax L Microplate Reader This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. keeping with best practices, reader performance should. Or scan up to 384 wells at any time to determine the best assay conditions. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate washer and reader solution: The SpectraMax® Mini Multi-Mode. Path Check Pathlength Measurement SpectraMax i3x. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. Molecular Devices: Molecular Devices. In addition, the SpectraMax iD5Next generation SpectraMax® absorbance microplate readers. Plate chamber design of the SpectraMax category 1 microplate readers. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. Devices’ StakMax® Microplate Stacker in a matter of minutes and . com 4. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SpectraMax Mini User Guide. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Fill a clean plate with water. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell. . Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. The chamber contains springs that position the cuvette in the proper alignment for a read. In addition, the SpectraMax iD5Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. 306 plate air cal. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SpectraMax 250 User. The advanced optical and. Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. Read one sample or up to 384 in0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. 2. 76358-624. The dual monochromator optics allow. In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The following document is intended to demonstrate setup of this instrument. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. 0 out of 5 $ 23,995 $ 22,495. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Add to Cart. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Italic and boldface type are used for emphasis. The powerful analysis tools in the software generate a standard curve and calculated data. 100 fmol 384-well (75 µL) 0. Maximum signal with lowest background. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. Promega's ROS-Glo H202 assay can be combined with SpectraMax readers to explore the relationship between. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The eight-channel system consists of 8 sample beams and 8 reference beams, delivering both superior precision and speed of reading across the microplate. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Fluorescence Microplate Reader Comparison Chart. Microplate Readers Biotek Epoch Microplate Reader. Sale. The optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. Table of Contents. Introduction. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Five preferred detection modes. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. . The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. 313 reference gain check fail. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. Download PDF. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. 6 for further experiment, so I'm confused which i would choose for OD value determination. For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Step 1: Turn on your microplate reader. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. 0 CART STOREVersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. 100 readings per well gives optimal Z’ values for this assay. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. Or, with the reader specifications noted from the Instrument Description section of your reader's. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. SpectraMax Spectrofluorometers. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. The screw remains inside the instrument. When the user applies a set temperature of 37°C, the top is heated to 37. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. 6% for the on-board optics (monochromators). The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 8 0112-0126D ComputerIntegration. ¨0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingThe SpectraMax iD5 Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. For more sample throughput, the system can SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. DNA and RNA measurements in SpectraMax® microplateSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. SpectraMax M5 reader settings are shown. Time-. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. We would like to show you a description here but the site won’t allow us. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 1. With optimized reagents, option to operate the. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. Products : General, Microplate Readers. 304 signal level saturation. SpectraMax® Microplate Readers and SoftMax® Pro Software. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. , April 14, 2022 – Molecular Devices, LLC. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. More Product Information. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. 7°C and the bottom to 36. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SoftMax® Pro 7. The analysis capabilities of the software provide quantitation in an easy-to-read, user customizable report format. We would like to show you a description here but the site won’t allow us. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. Access this by going to Help >. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Prerequisites: SoftMax Pro 7. The advanced optical and. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. For readers without a cuvette port, simply check the box next to ‘PathCheck’. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Products. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96-. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. The drawing shows a standard microplate of 15 mm height covered with a lid. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual. The temperature in the plate chamber is ambient and isothermal. With optimized reagents and the industry-leading data acquisition andSpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Absorbance reading with SpectraMax® microplate readers and SoftMax® Pro Software enables optical density measurement of samples in microplate or cuvette format. The cuvette must be in place when you read the plate. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today launched its. IMAP FP Settings in SoftMax Pro SpectraMax M5 Multi-Mode Microplate Reader. microplate reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. All . With optimized reagents, option to operate the. . The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. 40266 Email: drugdiscoverytech@lifetech. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. The plate. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. This technology allows for the automated regulation of well absorbance to a cuvette equivalent path length of 1 cm, which translates to. Table 1. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. For a higher . A: Microplate Preparation. Assays vary depending on. 7°C (gradient heating design). Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Microplate Readers BioTek Epoch 2 Microplate Reader. In addition, the SpectraMax iD5The SpectraMax® L Luminescence Microplate Reader is a high-performance microplate reader that measures luminescence in 96 and 384-well microplates. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. begin reading microplates with seven mouse clicks. Each sample has a discrete sample beam and reference beam so that each well is measured directly. The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. 316 grating motor driver faulty. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. Published Dec 16, 2020 | Updated May 19, 2023. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. Path Check Pathlength Measurement every Gemini EM Reader. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. The SpectraMax microplate readers can be used to accurately quantify ROS levels using a luminescence-based assay. Type:Spectrophotometer. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. It measures the optical density (OD) of samples in both 96- and 384-well. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. It also integrates with the. Tecan Infinite Microplate Reader. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. Wide range of applications The VersaMax reader covers. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 µL sample volume in a. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). For readers without a cuvette port, these options are not shown in the software. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. 4 μL DNA standards. These lab workhorses are used in drug discovery. 0 out of 5 $ 5,999. . A new technical approach to platelet aggregation studies. These robust readers have been placed in labs from. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. It can run samples based on pre-defined protocols and standard filter modes, and is compatible with ELISA and protein quantification assays. In addition, the SpectraMax® iD5 can be expanded to include bottom. Temperature control. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. 303 unable to cal dark current. An assay plate containing a 15-point standard curve was read on the SpectraMax. The SpectraMax L Luminescence Microplate Reader with the variable-volume injectors option (10 µL to 100 µL) can measure both flash and glow luminescence. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. The SpectraMax Quant dsDNA Assay Kits consist of three products, each designed for a specific range of DNA concentrations and optimized for use with fluorescence microplate readers such as the SpectraMax microplate reader (Table 1). Most papers they cultured bacteria to OD600>0. 997 (n = 3). Lysozyme contains two tryptophan residues that. edu) to be added as a trained user in iLab. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Molecular Devices. Nucleic Acid and Protein Quantitation. The Gemini XPS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. Table 2 summarizes the results for the uniformity test. Category 2 and 3 microplate readers use gradient heating as well. Microplate Readers Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMinimum volume and maximum throughput with 96- or 384-well microplate compatibility. Microplate Spectrophotometers. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. . The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. The SpectraMax iD3 reader is fully compatible with the SpectraDropTM Micro Volume Microplate, enabling quantitation of precious low volume samples. Wide range of applications. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. The calculated LOQ values were 0. . The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. B: Selection of Excitation Wavelength. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. SpectraMax Quant dsDNA Assay Kits are highly selective for dsDNA. 1. SpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. com. The SpectraMax iD3 reader allows you to interact with your data any way you want, from anywhere you want. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. Supplier: Molecular Devices. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. begin reading microplates with seven mouse clicks. microplate reader. 1 software Download page. SpectraMax Spectrofluorometers. 0 out of 5 $ 11,999. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. The screw remains inside the instrument. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. Intended Use/Discipline:Biological. Microplate reader assays requiring infrared (IR) or red-shifted. 1. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Microplate Automation & Detection. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile microplate reader capable of quantitating samples in a variety of microplate formats. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow toFlexStation® 3 Multi-Mode Microplate Reader.